Applied Biosystems™ HIV-1 Genotyping Kit with Integrase

The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase is a powerful tool for researchers investigating drug resistance mutations (DRMs) in the genetically diverse HIV-1 virus. This video series provides a comprehensive guide to using the kit, designed for both newcomers and experienced users who may need a refresher or assistance with specific steps in the workflow. It is an invaluable resource for anyone working with HIV-1 genotyping, particularly in studies of drug resistance to protease inhibitors (PI), nucleoside reverse-transcriptase inhibitors (NRTI), non-nucleoside reverse-transcriptase inhibitors (NNRTI), and integrase inhibitors (INSTI).

The training series is divided into segments that cover individual components of the workflow, ensuring users can access targeted information on specific tasks. The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase works by genotyping HIV-1 RNA extracted from EDTA plasma and dried blood spot samples. The video training will walk users through each step, from sample preparation to analysis, allowing for accurate identification of drug-resistant mutations. This reliable genotyping method uses Sanger sequencing, a time-tested technology that delivers precise results and is licensed by the U.S. Centers for Disease Control and Prevention (CDC).

One of the key advantages of this kit is its ability to detect mutations in the HIV-1 virus, a virus that evolves rapidly and is genetically diverse. By identifying these mutations, researchers can investigate the relationship between specific drug resistance mutations and treatment efficacy, paving the way for more targeted HIV therapies. The Sanger sequencing results provided by this kit are essential for understanding how the virus adapts to treatment and how to optimize drug regimens for patients.

It is important to note that this kit is intended for research use only and not for diagnostic procedures.